ID30A-3 / MASSIF-3

Contacts

Tel: +33 (0) 476 88 +ext

Control Cabin 2891

Igor Melnikov +33 (0) 476 20 4064
Beamline Scientist

Christoph Mueller-Dieckmann +33 (0) 476 88 2870
Scientist in charge

David Flot +33 (0) 476 88 1763
Beamline Operation Manager

Information for users

Synopsis

Fixed energy minifocus beamline

Status: open

Disciplines

Life Sciences

Applications

MASSIF-3 is a minifocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger1 X 4M detector that allows data collection at a very high frame rate (up to 750 Hz). The high resolution limit on the beamline is 1.17 Å.

Techniques

Anomalous diffraction
MX - macromolecular crystallography
Microbeam
Microcrystallography
Optical spectroscopy
Single-crystal diffraction
XRD - X-ray diffraction

Energy range

12.81 keV

Beam size

Minimum (H x V) : 15.0 x 15.0 µm²
Maximum (H x V) : 15.0 x 15.0 µm²

Sample environments

100 K cryostream
automatic sample changer
dehydration device
online microspec

Detectors

Eiger1 X 4M

Technical details

High resolution limit - 1.17Å

References:

ID30A-3 (MASSIF-3) – a beamline for macromolecular crystallography at the ESRF with a small intense beam, D. Von Stetten et al., J. Synchrotron Radiat. (2020) 27: 844–851. doi: 10.1107/S1600577520004002

The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

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Latest publications

View all publications

Mechanistic insights into substrate recognition of human nucleoside diphosphate kinase C based on nucleotide-induced structural changes

Amjadi R., Werten S., Lomada S.K., Baldin C., Scheffzek K., Dunzendorfer-Matt T., Wieland T.,
International Journal of Molecular Sciences 25, 9768-1-9768-19 (2024)

A potent agonist-based PROTAC targeting Pregnane X Receptor that delays colon cancer relapse

Bansard L., Laconde G., Delfosse V., Huet T., Ayeul M., Rigal E., Donati Q., Gerbal-Chaloin S., Daujat-Chavanieu M., Legrand B., Chavanieu A., Martin A.R., Pannequin J., Bourguet W., Amblard M., Pascussi J.M.,
BioRxiv , 062224 (2024)

PrgE: An OB-fold protein from plasmid pCF10 with striking differences to prototypical bacterial SSBs

Breidenstein A., Lamy A., Bader C.P.J., Sun W.S., Wanrooij P.H., Berntsson R.P.A.,
Life Science Alliance 7, e202402693-1-e202402693-14 (2024)

4'-ethynyl-2'-deoxycytidine (EdC) preferentially targets lymphoma and leukemia subtypes by inducing replicative stress

Calbert M.L., Chandramouly G., Adams C.M., Saez-Ayala M., Kent T., Tyagi M., Ayyadevara V.S.S.A., Wang Y., Krais J.J., Gordon J., Atkins J., Toma M.M., Betzi S., Boghossian A.S., Rees M.G., Ronan M.M., Roth J.A., Goldman A.R., Gorman N., Mitra R., Childers W.E., Graña X., Skorski T., Johnson N., Hurtz C., Morelli X., Eischen C.M., Pomerantz R.T.,
Molecular Cancer Therapeutics 23, 683-699 (2024)

Family GH157 enzyme exhibits broad linkage tolerance and a dual endo/exo-β-glucanase activity on β-glucans

Caseiro C., McGregor N.G.S., Alves V.D., Carvalho A.L., Romao M., Davies G.J., Fontes C.M.G.A., Bule P.,
International Journal of Biological Macromolecules 282, 137402-1-137402-17 (2024)

Bdellovibrio bacteriovorus uses chimeric fibre proteins to recognize and invade a broad range of bacterial hosts

Caulton S.G., Lambert C., Tyson J., Radford P., Al-Bayati A., Greenwood S., Banks E.J., Clark C., Till R., Pires E., Sockett R.E., Lovering A.L.,
Nature Microbiology 9, 214-227 (2024)

partners

European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.