The device is carefully optimized for short dead time (~ 2 ms) employing a home-built capillary flow-through cell. The data acquisition is hardware synchronized with the stopped-flow mixing sequence. A sample volume of about 1 ml per syringe is required for bubble-free loading of the sample. Typical mixing shots need 0.1 ml of each liquid. Further details about the setup can be found in the following references. The capillary head and  reservoirs are thermally isolated. By keeping the head and reservoirs at two different temperatures, a temperature jump can be induced with a few millisecond deadtime over the range 5 - 70 oC.

L. Matthews and T. Narayanan, Colloid Polym. Sci., 301, 721 (2023).

T. Narayanan, J. Gummel and M. Gradzielski,  in Advances in Planar Lipid Bilayers and Liposomes, 20, p 171-196, (Elsevier, 2014). [Reprint is available upon request].

Photos of the stopped-flow devices (SFM-400 and SFM-4000) installed at the beamline:

 

Schematic representation of the system:

stopped-flow_diagram2-resize400x324.gif

useful link:

http://www.bio-logic.fr

Manual (pdf)

 

back to the sample environment overview