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ID30A-3 / MASSIF-3

Contacts

Tel: +33 (0) 476 88 +ext

Control Cabin 2891

Igor Melnikov +33 (0) 476 20 4064
Beamline Scientist

Christoph Mueller-Dieckmann +33 (0) 476 88 2870
Scientist in charge

David Flot +33 (0) 476 88 1763
Beamline Operation Manager

Information for users

Synopsis

Fixed energy minifocus beamline
Status:  open

Disciplines

  • Life Sciences

Applications

  • MASSIF-3 is a minifocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger1 X 4M detector that allows data collection at a very high frame rate (up to 750 Hz). The high resolution limit on the beamline is 1.17 Å.

Techniques

  • Anomalous diffraction
  • MX - macromolecular crystallography
  • Microbeam
  • Microcrystallography
  • Optical spectroscopy
  • Single-crystal diffraction
  • XRD - X-ray diffraction

Energy range

  • 12.81  keV

Beam size

  • Minimum (H x V) : 15.0 x 15.0  µm²
  • Maximum (H x V) : 15.0 x 15.0  µm²

Sample environments

  • 100 K cryostream
  • automatic sample changer
  • dehydration device
  • online microspec

Detectors

  • Eiger1 X 4M

Technical details

High resolution limit - 1.17Å

 

References:

 ID30A-3 (MASSIF-3) – a beamline for macromolecular crystallography at the ESRF with a small intense beam, D. Von Stetten et al., J. Synchrotron Radiat. (2020) 27: 844–851. doi: 10.1107/S1600577520004002

 

 The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

 

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Latest publications

View all publications
4'-ethynyl-2'-deoxycytidine (EdC) preferentially targets lymphoma and leukemia subtypes by inducing replicative stress

Calbert M.L., Chandramouly G., Adams C.M., Saez-Ayala M., Kent T., Tyagi M., Ayyadevara V.S.S.A., Wang Y., Krais J.J., Gordon J., Atkins J., Toma M.M., Betzi S., Boghossian A.S., Rees M.G., Ronan M.M., Roth J.A., Goldman A.R., Gorman N., Mitra R., Childers W.E., Graña X., Skorski T., Johnson N., Hurtz C., Morelli X., Eischen C.M., Pomerantz R.T.,
Molecular Cancer Therapeutics 23, 683-699 (2024)

partners

European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.