ID23-2: Gemini - Macromolecular Crystallography

Synopsis

ID23-2 is a fixed energy beamline dedicated to MX. ID23-2 offers a standard MX sample environment, but with a focused microbeam (10x4 micron).

Status: open

Disciplines

Life Sciences

Applications

Macromolecular crystallography

Techniques

MX - macromolecular crystallography
Microbeam

Energy range

14.2 keV

Beam size

Minimum (H x V) : 1.0 x 2.0 µm²
Maximum (H x V) : 8.0 x 25.0 µm²

Sample environments

Microdiffractometer MD3Up
UNIPUCK+SPINE pins only (no SC3)

Detectors

DECTRIS EIGER X 9M

Technical details

This beamline offers diffraction data collection on microcrystalline MX samples and on fine needle MX samples, as well as determination of the region of highest diffraction quality from larger MX samples.

ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF

Max Nanao¹, Shibom Basu², Ulrich Zander², Thierry Giraud¹, John Surr¹, Matias Guijarro¹, Mario Lentini¹, Franck Felisaz², Jeremy Sinoir², Christian Morawe¹, Amparo Vivo¹, Antonia Beteva¹, Marcus Oscarsson¹, Hugo Caserotto¹, Fabien Dobias¹, David Flot¹, Didier Nurizzo¹, Jonathan Gigmes¹, Nicolas Foos¹, Ralf Siebrecht³, Thomas Roth¹, Pascal Theveneau¹, Olof Svensson¹, Gergely Papp¹, Bernard Lavault³, Florent Cipriani², Ray Barrett¹, Carole Clavel¹, Gordon Leonard¹

2022 Mar 1;29(Pt 2):581-590. doi: 10.1107/S1600577522000984

Latest publications

View all publications

Structure of a novel form of phosphopantetheine adenylyltransferase from Klebsiella pneumoniae at 2.59 Å resolution

Ahmad N., Sharma P., Sharma S., Singh T.P.,
European Biophysics Journal 53, 147-157 (2024)

An alternative conformation of the N-terminal loop of human dihydroorotate dehydrogenase drives binding to a potent antiproliferative agent

Alberti M., Poli G., Broggini L., Sainas S., Rizzi M., Boschi D., Ferraris D.M., Martino E., Ricagno S., Tuccinardi T., Lolli M.L., Miggiano R.,
Acta Crystallographica D 80, 386-396 (2024)

Mechanistic insights into substrate recognition of human nucleoside diphosphate kinase C based on nucleotide-induced structural changes

Amjadi R., Werten S., Lomada S.K., Baldin C., Scheffzek K., Dunzendorfer-Matt T., Wieland T.,
International Journal of Molecular Sciences 25, 9768-1-9768-19 (2024)

Conserved signal transduction mechanisms and dark recovery kinetic tuning in the Pseudomonadaceae short Light, Oxygen, Voltage (LOV) protein family

Arinkin V., Granzin J., Jaeger K.E., Willbold D., Krauss U., Batra-Safferling R.,
Journal of Molecular Biology 436, 168458-1-168458-20 (2024)

A potent agonist-based PROTAC targeting Pregnane X Receptor that delays colon cancer relapse

Bansard L., Laconde G., Delfosse V., Huet T., Ayeul M., Rigal E., Donati Q., Gerbal-Chaloin S., Daujat-Chavanieu M., Legrand B., Chavanieu A., Martin A.R., Pannequin J., Bourguet W., Amblard M., Pascussi J.M.,
BioRxiv , 062224 (2024)

Small-molecule positive allosteric modulation of homomeric kainate receptors GluK1-3: Development of screening assays and insight into GluK3 structure

Bay Y., Venskutonyté R., Frantsen S.M., Thorsen T.S., Musgaard M., Frydenvang K., Francotte P., Pirotte B., Biggin P.C., Kristensen A.S., Boesen T., Pickering D.S., Gajhede M., Kastrup J.S.,
FEBS Journal 291, 1506-1529 (2024)

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