Angeli A., Ferraroni M., Capasso C., Supuran C.T.,
Chemistry - An Asian Journal 19, e202400067-1-e202400067-6 (2024)
ID30B / MAD
Synopsis
ID30B is a highly automated, undulator-based, tunable beamline dedicated to macromolecular crystallography.Disciplines
- Life Sciences
Applications
- Life sciences
- Biological Science
- Biochemistry
- Structural biology
Techniques
- MAD - multi-wavelength anomalous dispersion
- MX - macromolecular crystallography
Energy range
- 6.0 - 20.0 keV
Beam size
- Minimum (H x V) : 20.0 x 20.0 µm²
- Maximum (H x V) : 200.0 x 200.0 µm²
Sample environments
- ID30B is equipped with a modified microdiffractometer (MD2-S), allowing data collection from micron-sized crystals mounted on conventional cryo-supports or in crystallisation plates (in situ).
- A new generation, flexible and high throughput sample changer (FlexHCD) capable of transferring samples from either EMBL/ESRF pucks or Unipucks has also been developed for ID30B in a collaboration between the ESRF and EMBL-Grenoble.
- An online Raman spectrometer is also available on request, please contact the iCOS staff to discuss the feasibility of the experiment well in advance.
Detectors
- EIGER2 X 9M 450µm Si sensor (Dectris). This detector was purchased by the ESRF.
- AXAS-A X-ray fluorescence detector (KETEK)
Technical references
McCarthy, A. A., Barrett, R., Beteva, A., Caserotto, H., Dobias, F., Felisaz, F., Giraud, T., Guijarro, M., Janocha, R., Khadrouche, A., Lentini, M., Leonard, G. A., Lopez Marrero, M., Malbet-Monaco, S., McSweeney, S., Nurizzo, D., Papp, G., Rossi, C., Sinoir, J., Sorez, C., Surr, J., Svensson, O., Zander, U., Cipriani, F., Theveneau, P., Mueller-Dieckmann, C. ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF. J. Synchrotron Radiat. 25,1249-1260 (2018).
Mueller-Dieckmann, C., Bowler, M. W., Carpentier, P., Flot, D., McCarthy, A. A., Nanao, M. H., Nurizzo, D., Pernot, P., Popov, A., Round, A., Royant, A., de Sanctis, D., von Stetten, D., Leonard, G. A. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility, Eur. Phys. J. Plus 130, 70 (2015).
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Latest publications
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Balasco N., Ruggiero A., Smaldone G., Pecoraro G., Coppola L., Pirone L., Pedone E.M., Esposito L., Berisio R., Vitagliano L.,
International Journal of Biological Macromolecules 277, 134390-1-134390-11 (2024)
Bancet A., Frem R., Jeanneret F., Mularoni A., Bazelle P., Roelants C., Delcros J.G., Guichou J.F., Pillet C., Coste I., Renno T., Battail C., Cochet C., Lomberget T., Filhol O., Krimm I.,
iScience 27, 108903-1-108903-23 (2024)
Bárria C., Athayde D., Hernandez G., Fonseca L., Casinhas J., Cordeiro T.N., Archer M., Arraiano C.M., Brito J.A., Matos R.G.,
Biochimie 216, 56-70 (2024)
Beck J., Shanmugaratnam S., Höcker B.,
Protein Science 33, e5001-1-e5001-11 (2024)
Boverio A., Jamil N., Mannucci B., Mascotti M.L., Fraaije M.W., Mattevi A.,
Nature Communications 15, 4158-1-4158-10 (2024)