Methods
Spectroscopic Methods
The Cryobench laboratory offers...
- UV/vis absorption spectroscopy,
- fluorescence (emission) spectroscopy,
- fluorescence lifetime measurements,
- Raman spectroscopy,
...of protein crystals mounted on a goniometer between three objectives, usually cooled by a cryogenic stream.
Additionally, it is possible to perform spectroscopic experiments directly at X-ray beamlines:
- online UV/vis and fluorescence spectroscopy (available twice per year),
- online Raman spectroscopy at beamline ID29 (available upon request).
For typical experiments in this lab, standard protocols have been defined (intranet only). Suggestions for solving some frequent problems which you may encounter during your experiment can be found here.
Make sure that you know the safety regulations before coming to the Cryobench lab!