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Publications

2023

Experimental data

  1. Bui S, Gil-Guerrero S, van der Linden P, Carpentier P, Ceccarelli M, Jambrina PG, Steiner RA. "Evolutionary adaptation from hydrolytic to oxygenolytic catalysis at the α/β-hydrolase fold." (2023) Chem Sci. 14, 10547-10560. [online microspec]
  2. Kovalev K, Tsybrov F, Alekseev A, Shevchenko V, Soloviov D, Siletsky S, Bourenkov G, Agthe M, Nikolova M, von Stetten D, Astashkin R, Bukhdruker S, Chizhov I, Royant A, Kuzmin A, Gushchin I, Rosselli R, Rodriguez-Valera F, Ilyinskiy N, Rogachev A, Borshchevskiy V, Schneider TR, Bamberg E, Gordeliy V. "Mechanisms of inward transmembrane proton translocation." (2023) Nat Struct Mol Biol. 30, 970-979. [UV-vis Absorption]

2022

Experimental data

  1. Albert L, Steinchen W, Werel L, Nagpal J, Djokovic N, Ruzic D, Hoffarth M, Xu J, Royant A, Bange G, Nikolic K, Ryu S, Dou Y, Essen LO, Olalla Vázquez O. "In Vivo Optochemical Control of Hematopoiesis by MLL1 Complex Disruption." (2022) ACS Cent. Sci. 8, 57-56 [UV-vis Absorption]
  2. Astashkin R, Kovalev K, Bukhdruker S, Vaganova S, Kuzmin A, Alekseev A, Balandin T, Zabelskii D, Gushchin I, Royant A, Volkov D, Bourenkov G, Koonin E, Engelhard M, Bamberg E, Gordeliy V. "Structural insights into light-driven anion pumping in cyanobacteria." (2022) Nat. Commun. 13:6460 [UV-vis Absorption]
  3. Aumonier S, Engilberge S, Caramello C, Gotthard G, Leonard GA, Mueller-Dieckmann C, Royant A. "Slow protein dynamics probed by time-resolved oscillation crystallography at room temperature." (2022) IUCrJ, in press. [UV-vis Absorption]
  4. Borshchevskiy V, Kovalev K, Round E, Efremov R, Astashkin R, Bourenkov G, Bratanov D, Balandin T, Chizhov I, Baeken C, Gushchin I, Kuzmin A, Alekseev A, Rogachev A, Willbold D, Engelhard M, Bamberg E, Büldt G, Gordeliy V. ''True-atomic-resolution insights into the structure and functional role of linear chains and low-barrier hydrogen bonds in proteins.'' (2022) Nat. Struct. Mol. Biol. 29, 440-450. [UV-vis Absorption]
  5. van der Linden P, Engilberge S, Atta M, Carpentier P. "A miniature airlock system to aid the cryo-cooling of protein crystals grown under anoxic conditions." (2022) J. Appl. Crystallogr. 55, 1212-1218. [UV-vis Absorption]
  6. Maestre-Reyna M, Yang CH, Nango E, Huang WC, Ngurah Putu EPG, Wu WJ, Wang PH, Franz-Badur S, Saft M, Emmerich HJ, Wu HY, Lee CC, Huang KF, Chang YK, Liao JH, Weng JH, Gad W, Chang CW, Pang AH, Sugahara M, Owada S, Hosokawa Y, Joti Y, Yamashita A, Tanaka R, Tanaka T, Luo F, Tono K, Hsu KC, Kiontke S, Schapiro I, Spadaccini R, Royant A, Yamamoto J, Iwata S, Essen LO, Bessho Y, Tsai MD.  "Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme.'' (2022) Nat. Chem. 14, 677-685. [UV-vis Absorption]
  7. Rodrigues MJ, Giri N, Royant A, Zhang Y, Bolton R, Evans G, Ealick SE, Begley T, Tews I. ''Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase.'' (2022) RSC Chem. Biol. 3, 227-230. [UV-vis Absorption]

2021

Experimental data

  1. Arinkin V, Granzin J, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R. ''Structural determinants underlying the adduct lifetime in the LOV proteins of Pseudomonas putida.'' (2021) FEBS J. 16, 4955-4972. [UV-vis Absorption]
  2. Bou-Nader C, Stull FW, Pecqueur L, Simon P, Guérineau V, Royant A, Fontecave M, Lombard M, Palfey BA, Hamdane D. ''An enzymatic activation of formaldehyde for nucleotide methylation." (2021) Nat Commun. 12:4542. [UV-vis Absorption]
  3. Chiari L, Carpentier P, Kieffer-jaquinod S,  Gogny A, Perard J, Ravanel S, Cobessi D, Menage S, Dumas R, Hamelin O. ''LEAFY protein crystals with a honeycomb structure as platform for selective preparation of outstanding stable bio-hybrid materials.'' (2021) Nanoscale. 20, 8901-8908. [UV-vis Absorption / Raman]
  4. Sorigué D, Hadjidemetriou K, Blangy S, Gotthard G, Bonvalet A, Coquelle N, Samire P, Aleksandrov A, Antonucci L, Benachir A, Boutet S, Byrdin M, Cammarata M, Carbajo S, Cuiné S, Doak RB, Foucar L, Gorel A, Grünbein M, Hartmann E, Hienerwadel R, Hilpert M, Kloos M, Lane TJ, Légeret B, Legrand P, Li-Beisson Y, Moulin SLY, Nurizzo D, Peltier G, Schirò G, Shoeman RL, Sliwa M, Solinas X, Zhuang B, Barends TRM, Colletier JP, Joffre M, Royant A, Berthomieu C, Weik M, Domratcheva T, Brettel K, Vos MH, Schlichting I, Arnoux P, Müller P, Beisson F. ''Mechanism and dynamics of fatty acid photodecarboxylase.'' (2021) Science. 9, 372(6538):eabd5687. [UV-vis Absorption / Raman]

2020

Experimental data

  1. Aumonier S, Santoni G, Gotthard G, von Stetten D, Leonard GA, Royant A. ''Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron.'' (2020) IUCrJ 7, 728-736. [UV-vis Absorption]
  2. Carpentier P, Leprêtre C, Basset C, Douki T, Torelli S, Duarte V, Hamdane D, Fontecave M, Atta M. ''Structural, biochemical and functional analyses of tRNA-monooxygenase enzyme MiaE from Pseudomonas putida provide insights into tRNA/MiaE interaction''. (2020) Nucleic Acids Res. 48, 9918-9930. [UV-vis Absorption]
  3. De Zitter E, Ridard J, Thédié D, Adam V, Lévy B, Byrdin M, Gotthard G, Van Meervelt L, Dedecker P, Demachy I, Bourgeois D. ''Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State.'' (2020) J. Am. Chem. Soc. 142, 10978-10988. [Raman]
  4. Kovalev K, Astashkin R, Gushchin I, Orekhov P, Volkov D, Zinovev E, Marin E, Rulev M, Alekseev A, Royant A, Carpentier P, Vaganova S, Zabelskii D, Baeken C, Sergeev I, Balandin T, Bourenkov G, Carpena X, Boer R, Maliar N, Borshchevskiy V, Büldt G, Bamberg E, Gordeliy V. "Molecular mechanism of light-driven sodium pumping.'' (2020) Nat. Commun. 11:2137. [UV-vis Absorption]
  5. Kovalev K, Volkov D, Astashkin R, Alekseev A, Gushchin I, Haro-Moreno JM, Chizhov I, Siletsky S, Mamedov M, Rogachev A, Balandin T, Borshchevskiy V, Popov A, Bourenkov G, Bamberg E, Rodriguez-Valera F, Büldt G, Gordeliy V. "High-resolution structural insights into the heliorhodopsin family.'' (2020) Proc. Natl. Acad. Sci. USA 117, 4131-4141. [UV-vis Absorption]
  6. Xu X, Port A, Wiebeler C, Zhao KH, Schapiro I, Gärtner W. ''Structural elements regulating the photochromicity in a cyanobacteriochrome.'' (2020) Proc. Natl. Acad. Sci. USA 117, 2432-2440. [UV-vis Absorption]

2019

Experimental data

  1. Ardiccioni C, Arcovito A, Della Longa S, van der Linden P, Bourgeois D, Weik M, Montemiglio LC, Savino C, Avella G, Exertier C, Carpentier P, Prangé T, Brunori M, Colloc'h N, Vallone B. "Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments.'' (2019) IUCrJ 6, 832-842. [UV-vis Absorption]
  2. Chaplin AK, Chicano TM, Hampshire BV, Wilson MT, Hough MA, Svistunenko DA, Worrall JAR. ''An Aromatic Dyad Motif in Dye Decolourising Peroxidases Has Implications for Free Radical Formation and Catalysis.'' (2019) Chemistry 25, 6141-6153. [UV-vis Absorption] 
  3. Gotthard G, Aumonier S, De Sanctis D, Leonard G, von Stetten D, Royant A. ''Specific radiation damage is a lesser concern at room temperature''. (2019) IUCrJ 6, 665-680. [UV-vis Absorption/Raman]
  4. Kovalev K, Polovinkin V, Gushchin I, Alekseev A, Shevchenko V, Borshchevskiy V, Astashkin R, Balandin T, Bratanov D, Vaganova S, Popov A, Chupin V, Büldt G, Bamberg E, Gordeliy V. ''Structure and mechanisms of sodium-pumping KR2 rhodopsin.'' (2019) Sci. Adv. 5:eaav2671. [UV-vis Absorption]
  5. Torra J, Lafaye C, Signor L, Aumonier S, Flors C, Shu X, Nonell S, Gotthard G, Royant A. ''Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein.'' (2019) Sci. Rep. 9:2428. [UV-vis Absorption/Fluorescence]

Review or citation

  1. Ash PA, Kendall-Price SET, Vincent KA. ''Unifying Activity, Structure, and Spectroscopy of [NiFe] Hydrogenases: Combining Techniques To Clarify Mechanistic Understanding.'' (2019) Acc. Chem. Res. 19, 3120-313.
  2. Kandiah E, Giraud T, de Maria Antolinos A, Dobias F, Effantin G, Flot D, Hons M, Schoehn G, Susini J, Svensson O, Leonard GA, Mueller-Dieckmann C. ''CM01: a facility for cryo-electron microscopy at the European Synchrotron.'' (2019) Acta Crystallogr D Struct Biol. 75, 528-535.
  3. Liang J, Ye X, He J, Liu J, Huang Y, Xing F. ''Novel Immune Microlens Imaging for Detection of Antigen and Antibody.'' (2019) J Immunol Res. 18, 5474519. 
  4. Smulevich G. ''Solution and crystal phase resonance Raman spectroscopy: Valuable tools to unveil the structure and function of heme proteins'' (2019)  Journal of Porphyrins and Phthalocyanines 23, 691-700.
  5. Tooke CL, Hinchliffe P, Bragginton EC, Colenso CK, Hirvonen VHA, Takebayashi Y, Spencer J. ''β-Lactamases and β-Lactamase Inhibitors in the 21st Century.'' (2019) J Mol Biol. 23, 3472-3500.

2018

Experimental data

  1. Gourinchas G, Heintz U, Winkler A. ''Asymmetric activation mechanism of a homodimeric red light‐regulated photoreceptor.'' (2018) Elife 7:e34815.  [UV-vis Absorption/Fluorescence]

2017

Experimental data

  1. Arinkin V, Granzin J, Röllen K, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R. “Structure of a LOV protein in apo-state and implications for construction of LOV-based optical tools.” (2017) Sci Rep. 7, 42971. [UV-vis Absorption/Fluorescence]
  2. Genovese I, Fiorillo A, Ilari A, Masciarelli S, Fazi F, Colotti G. ''Binding of doxorubicin to Sorcin impairs cell death and increases drug resistance in cancer cells.'' (2017) Cell Death Dis. 8:e2950. [Fluorescence]
  3. Gotthard G, von Stetten D, Clavel D, Noirclerc-Savoye M, Royant A. ''Chromophore isomer stabilization is critical to the efficient fluorescence of Cyan Fluorescent Proteins.'' (2017) Biochemistry 56, 6418-6422. [UV-vis Absorption/Fluorescence/Raman]
  4. Kekilli D, Moreno-Chicano T, Chaplin AK, Horrell S, Dworkowski FSN, Worrall JAR, Strange RW, Hough MA. “Photoreduction and validation of haem-ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction.” (2017) IUCrJ 4, 263-270. [online UV-vis Absorption]
  5. Rodrigues M, Windeisen V, Zhang Y, Guedez G, Weber S, Strohmeier M, Hanes J, Royant A, Evans G, Sinning I, Ealick S, Begley T, Tews I “Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis.” (2017) Nat. Chem. Biol. 13, 290-294. [(online) UV-vis Absorption]
  6. Romero E, Castellanos JR, Mattevi A, Fraaije MW. “Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase.” (2017) Angew. Chem. Int. Ed. 55, 15852-15855. [(online) UV-vis Absorption]
  7. Von Stetten D, Giraud T, Bui S, Steiner RA, Fihman F, de Sanctis D & Royant A. ''Online Raman spectroscopy for structural biology on beamline ID29 of the ESRF.'' (2017) J. Struct. Biol. 200, 124-127. [Raman]

Review or citation

  1. Ash PA, Carr SB, Reeve HA, Skorupskaitė A, Rowbotham JS, Shutt R, Frogley MD, Evans RM, Cinque G, Armstrong FA, Vincent KA. ''Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal.'' (2017) Chem. Commun. 30, 5858-5861.
  2. Horrell S, Kekilli D, Strange RW, Hough MA. ''Recent structural insights into the function of copper nitrite reductases.'' (2017) Metallomics. 15, 1470-1482.
  3. Hough MA. ''Choosing the optimal spectroscopic toolkit to understand protein function.'' (2017) Biosci Rep. 8:BSR20160378.
  4. Kwon H, Smith O, Raven EL, Moody PC. ''Combining X-ray and neutron crystallography with spectroscopy.'' (2017) Acta Crystallogr D Struct Biol. 73, 141-147.

2016

Experimental data

  1. Clavel D, Gotthard G, von Stetten D, De Sanctis D, Pasquier H, Lambert GG, Shaner NC, Royant A. "Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution." (2016). Acta Cryst. D72, 1298-1307. [UV-vis Absorption/Online Raman]
  2. Colletier JP, Sliwa M, Gallat FX, Sugahara M, Guillon V, Schirò G, Coquelle N, Woodhouse J, Roux L, Gotthard G, Royant A, Uriarte LM, Ruckebusch C, Joti Y, Byrdin M, Mizohata E, Nango E, Tanaka T, Tono K, Yabashi M, Adam V, Cammarata M, Schlichting I, Bourgeois D, Weik M. "Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP." (2016) J Phys Chem Lett. 7 (5), 882-887. [UV-vis Absorption]
  3. Esmieu C, Orio M, Le Pape L, Lebrun L, Pécaut J, Menage S, Torelli S. ''Redox-Innocent Metal-Assisted Cleavage of S–S Bond in a Disulfide-Containing Ligand.'' (2016) Inorg. Chem. 55, 6208-6217. [UV-vis Absorption/Raman]

Review or citation

  1. Cohen AE, Doukov T, Soltis MS. "UV-Visible Absorption Spectroscopy Enhanced X-ray Crystallography at Synchrotron and X-ray Free Electron Laser Sources" (2016) Protein and Peptide Letters. 23:3, 283-290.
  2. Owen RL, Juanhuix J, Fuchs M. "Current advances in synchrotron radiation instrumentation for MX experiments" (2016) Archives of Biochemistry and Biophysics. 602, 21-31.

2015

Experimental data

  1. Gushchin I, Shevchenko V, Polovinkin V, Kovalev K, Alekseev A, Round E, Borshchevskiy V, Balandin T, Popov A, Gensch T, Fahlke C, Bamann C, Willbold D, Büldt G, Bamberg E, Gordeliy V. “Crystal structure of a light-driven sodium pump.” (2015) Nat Struct Mol Biol. [UV-vis Absorption]
  2. Scheerer P, Zhang F, Kalms J, von Stetten D, Krauss N, Oberpichler I, Lamparter T. “The Class III Cyclobutane Pyrimidine Dimer Photolyase Structure Reveals a New Antenna Chromophore Binding Site and Alternative Photoreduction Pathways” (2015) J. Biol. Chem. Mar 17. [UV-vis Absorption]
  3. Sousa CM, Carpentier P, Matias PM, Testa F, Pinho F, Sarti P, Giuffrè A, Bandeiras TM, Romão CV. "Superoxide reductase from Giardia intestinalis: structural characterization of the first SOR from a eukaryotic organism shows an iron centre that is highly sensitive to photoreduction." (2015) Acta Crystallogr D Biol Crystallogr. 71, 2236-2247. [online microspec]
  4. Taberman H, Andberg M, Koivula A. "Structure and function of Caulobacter crescentus aldose-aldose oxidoreductase" (2015) Biochem J. 472, 297-307. [UV-vis Absorption]
  5. von Stetten D, Giraud T, Carpentier P, Sever F, Terrien M, Dobias F, Juers DH, Flot D, Mueller-Dieckmann C, Leonard GA, de Sanctis D and Royant A. “In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF” (2015) Acta Cryst. D71, 15-26. [UV-vis Absorption/Fluorescence/Raman]

Review or citation

  1. Bowler M, Mueller U, Weiss M, Sanchez-Weatherby J, Sorensen T, Thunnissen M, Ursby T, Gobbo A, Russi S, Bowler MG,Brockhauser S, Svensson O, and Cipriani F “Automation and Experience of Controlled Crystal Dehydration: Results from the European Synchrotron HC1 Collaboration” (2015) Cryst. Growth Des., 15 (3), pp 1043–1054
  2. Kekilli D, Dworkowski FS, Pompidor G, Fuchs MR, Andrew CR, Antonyuk S, Strange RW, Eady RR, Hasnain SS, Hough MA. “Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data.” (2015) Acta Crystallogr D71, 27-35.
  3. Mueller-Dieckmann C, Bowler MW, Carpentier P. "The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility" (2015) European Physical Journal Plus, 130, 4, 70.
  4. Pearson AR, von Stetten D, Huse N. "If You Can Get a Crystal Structure, Why Bother with Anything Else?" (2015), Synchrotron Radiation News, 28:6, 10-14.
  5. Sakaguchi M, Kimura T, Nishida T. "A nearly on-axis spectroscopic system for simultaneously measuring UV-visible absorption and X-ray diffraction in the SPring-8 structural genomics beamline" (2015) 2nd Workshop on FEL Photon Diagnostics, Instrumentation and Beamlines Design (PhotonDiag). J Synch Rad, 23, 334-338.

2014

Experimental data

  1. Bonnot F, Tremey E, von Stetten D, Rat S, Duval S, Carpentier P, Clemancey M, Desbois A, Nivière V. “Formation of high-valent iron-oxo species in superoxide reductase: characterization by resonance Raman spectroscopy.” (2014) Angew Chem Int Ed Engl. 53, 5926-30. [Raman]
  2. Borshchevskiy V, Round E, Erofeev I, Weik M, Ishchenko A, Gushchin I, Mishin A, Willbold D, Büldt G, Gordeliy V. “Low-dose X-ray radiation induces structural alterations in proteins.” (2014) Acta Crystallogr D70, 2675-85 [on-line UV-vis Absorption]
  3. Soi Bui, David von Stetten, Pablo G. Jambrina, Thierry Prangé, Nathalie Colloc’h, Daniele de Sanctis, Antoine Royant, Edina Rosta, and Roberto A. Steiner. "Direct Evidence for a Peroxide Intermediate and a Reactive Enzyme–Substrate–Dioxygen Configuration in a Cofactor-free Oxidase." (2014) Angew Chem Int Ed. 53, 13710-13714. [on-line Raman]
  4. Gudmundsson M, Kim S, Wu M, Ishida T, Momeni MH, Vaaje-Kolstad G, Lundberg D, Royant A, Ståhlberg J, Eijsink VG, Beckham GT, Sandgren M. “Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-ray Photoreduction.” (2014) J Biol Chem. 289, 18782-92. [on-line UV-vis Absorption]
  5. Jørgensen R, Batot G, Mannerstedt K, Imberty A, Breton C, Hindsgaul O, Royant A, Palcic MM. “Structures of a human blood group glycosyltransferase in complex with a photo-activatable UDP-Gal derivative reveal two different binding conformations.” (2014) Acta Crystallogr F Struct Biol Commun. 70, 1015-21. [UV-vis Absorption/Actinic]
  6. Polovinkin V, Balandin T, Volkov O, Round E, Borshchevskiy V, Utrobin P, von Stetten D, Royant A, Willbold D, Arzumanyan G, Chupin V, Popot JL, Gordeliy V. “Nanoparticle Surface-Enhanced Raman Scattering of Bacteriorhodopsin Stabilized by Amphipol A8-35.” (2014) J Membr Biol. [UV-vis Absorption/Raman]
  7. Shevchenko V, Gushchin I, Polovinkin V, Round E, Borshchevskiy V, Utrobin P, Popov A, Balandin T, Büldt G, Gordeliy V. “Crystal structure of Escherichia coli-expressed Haloarcula marismortui bacteriorhodopsin I in the trimeric form.” (2014) PLoS One 9, e112873 [UV-vis Absorption]
  8. Szczepek M, Beyrière F, Hofmann KP, Elgeti M, Kazmin R, Rose A, Bartl FJ, von Stetten D, Heck M, Sommer ME, Hildebrand PW, Scheerer P. “Crystal structure of a common GPCR-binding interface for G protein and arrestin” (2014) Nat. Commun. 5, 4801.  [UV-vis Absorption]

Review or citation

  1. Calero G, Cohen AE, Luft JR. "Identifying, studying and making good use of macromolecular crystals" (2014) Acta Crystallogr F Struct Biol Commun. 70, 993-1008.
  2. Dworkowski FS, Hough MA, Pompidor G, Fuchs MR. “Fingerprinting redox and ligand states in haemprotein crystal structures using resonance Raman spectroscopy.” (2014) Acta Crystallogr D70, 1289-96.
  3. Frankaer CG, Mossin S, Stahl K. "Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T-6 bovine insulin derivatives." Acta Crystallogr D70, 110-122.
  4. van Oort B, Kargul J, Barber J, van Amerongen H. “Fluorescence kinetics of PSII crystals containing Ca(2+) or Sr(2+) in the oxygen evolving complex.” (2014) Biochim Biophys Acta. 1837, 264-9.

2013

Experimental data

  1. Anders K, Daminelli-Widany G, Mroginski MA, von Stetten D, Essen LO. “Structure of the cyanobacterial phytochrome 2 photosensor implies a tryptophan switch for phytochrome signalling” (2013) J. Biol. Chem. 288, 35714-35725. [(on-line) UV-vis Absorption/Raman].
  2. Duan C, Adam V, Byrdin M, Ridard J, Kieffer-Jaquinod S, Morlot C, Arcizet D, Demachy I, Bourgeois D. "Structural evidence for a two-regime photobleaching mechanism in a reversibly switchable fluorescent protein." (2013) J. Am. Chem. Soc. 135, 15841-15850. [Raman]

Review or citation

  1. Barbosa P, Pedro J, Royant A, Panjikar S. “In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures.” (2013) J. Struct. Biol. 181, 89-94.
  2. Pompidor G, Dworkowski FS, Thominet V, Schulze-Briese C, Fuchs MR. “A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source.” (2013) J Synchrotron Radiat. 20, 765-76.
  3. Russo Krauss I, Merlino A, Vergara A, Sica F. “An overview of biological macromolecule crystallization.” (2013) Int J Mol Sci. 14, 11643-91.
  4. Theveneau P, Baker R, Barrett R. “The Upgrade Programme for the Structural Biology beam lines at the European Synchrotron Radiation Facility - High throughput sample evaluation and automation” (2013). Journal of Physics Conference Series 425, 012001.

2012

Experimental data

  1. Ferraroni M, Matera I, Chernykh A, Kolomytseva M, Golovleva LA, Scozzafava A, Briganti F. "Reaction intermediates and redox state changes in a blue laccase from Steccherinum ochraceum observed by crystallographic high/low X-ray dose experiments." (2012) J. Inorg. Biochem. 111, 203-209. [on-line UV-vis Absorption]
  2. Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TWJ, Royant A. "Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%." (2012) Nature Commun. 3, 751. [UV-vis Absorption/Fluorescence/TCSPC]
  3. De la Mora E, Lovett JE, Blanford CF, Garman EF, Valderrama B, Rudino-Pinera E. "Structural changes caused by radiation-induced reduction and radiolysis: the effect of X-ray absorbed dose in a fungal multicopper oxidase." (2012) Acta Cryst. D68, 564-577. [on-line UV-vis Absorption]
  4. de Rosny E, Carpentier P. "GFP-like phototransformation mechanisms in the cytotoxic fluorescent protein KillerRed unraveled by structural and spectroscopic investigations." (2012) J. Am. Chem. Soc. 134, 18015-18021. [(on-line) UV-vis Absorption/Fluorescence]
  5. von Stetten D, Noirclerc-Savoye M, Goedhart J, Gadella TWJ, Royant A. "Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K." (2012) Acta Cryst. F68, 878-882. [UV-vis Absorption/Fluorescence]
  6. von Stetten D, Batot GO, Noirclerc-Savoye M, Royant A. "Alteration of fluorescent protein spectroscopic properties upon cryoprotection." (2012) Acta Cryst. D68, 1578-1583. [UV-vis Absorption/Fluorescence]
  7. Wahlgren WY, Omran H, von Stetten D, Royant A, van der Post S, Katona G. "Structural Characterization of Bacterioferritin from Blastochloris viridis." (2012) PLOS ONE 7, e46992. [UV-vis Absorption]

Review or citation

  1. Camara-Artigas A, Bacarizo J, Andujar-Sanchez M. "pH-dependant structural conformations of B-phycoerythrin from Porphyridium cruentum." (2012) FEBS J. 279, 3680-3691.
  2. Kung Y, Ando N, Doukov TI, Blasiak LC, Bender G, Seravalli J, Ragsdale SW, Drennan CL. "Visualizing molecular juggling within a B12-dependent methyltransferase complex." (2012) Nature 484, 265-269.
  3. Owen RL, Yorke BA, Pearson AR. "X-ray-excited optical luminescence of protein crystals: a new tool for studying radiation damage during diffraction data collection." (2012) Acta Cryst. D68, 505-510.
  4. Stojanoff V, Northrup P, Pietri R, Zhong Z. "Synchrotron radiation in life sciences." (2012) Protein Pept. Lett. 19, 761-769.

2011

Experimental data

  1. Choe HW, Kim YJ, Park JH, Morizumi T, Pai EF, Krauß N, Hofmann KP, Scheerer P, Ernst OP. "Crystal structure of metarhodopsin II." (2011) Nature 471, 651-655. [UV-vis Absorption]
  2. Gumiero A, Metcalfe CL, Pearson AR, Raven EL, Moody PC. "Nature of the ferryl heme in compounds I and II." (2011) J. Biol. Chem. 286, 1260-1268. [on-line UV-vis Absorption]
  3. Hersleth HP, Andersson KK. "How different oxidation states of crystalline myoglobin are influenced by X-rays." (2011) Biochim. Biophys. Acta 1814, 785-796. [on-line UV-vis Absorption]
  4. Kiontke S, Geisselbrecht Y, Pokorny R, Carell T, Batschauer A, Essen LO. "Crystal structures of an archaeal class II DNA photolyase and its complex with UV-damaged duplex DNA." (2011) EMBO J. 30, 4437-4449. [on-line UV-vis Absorption]
  5. Mailliet J, Psakis G, Feilke K, Sineshchekov V, Essen LO, Hughes J. "Spectroscopy and a high-resolution crystal structure of Tyr263 mutants of cyanobacterial phytochrome Cph1." (2011) J. Mol. Biol. 413, 115-127. [UV-vis Absorption/Fluorescence]
  6. McGeehan JE, Bourgeois D, Royant A, Carpentier P. "Raman-assisted crystallography of biomolecules at the synchrotron: Instrumentation, methods and applications." (2011) Biochim. Biophys. Acta 1814, 750-759. [on-line Raman]
  7. Orru R, Dudek HM, Martinoli C, Torres Pazmiño DE, Royant A, Weik M, Fraaije MW, Mattevi A. "Snapshots of enzymatic Baeyer-Villiger catalysis: oxygen activation and intermediate stabilization." (2011) J. Biol. Chem. 286, 29284-29291. [(on-line) UV-vis Absorption]
  8. Rajagopal BS, Wilson MT, Bendall DS, Howe CJ, Worrall JAR. "Structural and kinetic studies of imidazole binding to two members of the cytochrome c6 family reveal an important role for a conserved heme pocket residue." (2011) J. Biol. Inorg. Chem. 16, 577-588. [UV-vis Absorption]
  9. Regis-Faro A, Carpentier P, Jonasson G, Pompidor G, Arcizet D, Demachy I, Bourgeois D. "Low-temperature chromophore isomerization reveals the photoswitching mechanism of the fluorescent protein Padron." (2011) J. Am. Chem. Soc. 133, 16362-16365. [(on-line) UV-vis Absorption/Fluorescence]
  10. Royant A, Noirclerc-Savoye M. "Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein." (2011) J. Struct. Biol. 174, 385-390. [UV-vis Absorption/Fluorescence/TCSPC]
  11. Trepreau J, Girard E, Maillard AP, de Rosny E, Petit-Haertlein I, Kahn R, Covès J. "Structural basis for metal sensing by CnrX." (2011) J. Mol. Biol. 408, 766-779. [UV-vis Absorption]
  12. Vinzenz X, Grosse W, Linne U, Meissner B, Essen LO. "Chemical engineering of Mycobacterium tuberculosis dodecin hybrids." (2011) Chem. Commun. 47, 11071-11073. [UV-vis Absorption/Fluorescence]

Review or citation

  1. Arcizet D, Adam V, Carpentier P, Faro A, Bourgeois D. "Combining kinetic crystallography and optical spectroscopy for studying fluorescent proteins." (2011) Actualité Chimique 356-57, 78-83.
  2. Bettati S, Pasqualetto E, Lolli G, Campanini B, Battistutta R. "Structure and single crystal spectroscopy of Green Fluorescent Proteins." (2011) Biochim. Biophys. Acta 1814, 824-833.
  3. Carey PR, Chen Y, Gong B, Kalp M. "Kinetic crystallography by Raman microscopy." (2011) Biochim. Biophys. Acta 1814, 742-749.
  4. Juers DH, Weik M. "Similarities and differences in radiation damage at 100 K versus 160 K in a crystal of thermolysin." (2011) J. Synchrotron Rad. 18, 329-337.
  5. Karuppasamy M, Nejadasl FK, Vulovic M, Kostera AJ, Ravelli RBG. "Radiation damage in single-particle cryo-electron microscopy: effects of dose and dose rate." (2011) J. Synchrotron Rad. 18, 398-412.
  6. Kmetko J, Warkentin M, Englich U, Thorne RE. "Can radiation damage to protein crystals be reduced using small-molecule compounds?" (2011) Acta Cryst. D67, 881-893.
  7. Orville AM, Buono R, Cowan M, Héroux A, Shea-McCarthy G, Schneider DK, Skinner JM, Skinner MJ, Stoner-Ma D, Sweet RM. "Correlated single-crystal electronic absorption spectroscopy and X-ray crystallography at NSLS beamline X26-C." (2011) J. Synchrotron Rad. 18, 358-366.
  8. Owen RL, Yorke BA, Gowdy JA, Pearson AR. "Revealing low-dose radiation damage using single-crystal spectroscopy." (2011) J. Synchrotron Rad. 18, 367-373.
  9. Ronda L, Bruno S, Bettati S, Mozzarelli A. "Protein crystal microspectrophotometry." (2011) Biochim. Biophys. Acta 1814, 734-741.
  10. Sage JT, Zhang Y, McGeehan J, Ravelli R, Weik M, van Thor JJ. "Infrared protein crystallography." (2011) Biochim. Biophys. Acta 1814, 760-777.
  11. Stoner-Ma D, Skinner JM, Schneider DK, Cowan M, Sweet RM, Orville AM. "Single-crystal Raman spectroscopy and X-ray crystallography at beamline X26-C of the NSLS." (2011) J. Synchrotron Rad. 18, 37-40.

2010

Experimental data

  1. Bento I, Silva CS, Chen Z, Martins LO, Lindley PF, Soares CM. "Mechanisms underlying dioxygen reduction in laccases. Structural and modelling studies focusing on proton transfer." (2010) BMC Struct. Biol. 10:28. [UV-vis Absorption]
  2. Carpentier P, Royant A, Weik M, Bourgeois D. "Raman-assisted crystallography suggests a mechanism of x-ray-induced disulfide radical formation and reparation." (2010) Structure 18, 1410-1419. [on-line Raman]
  3. Cavazza C, Bochot C, Rousselot-Pailley P, Carpentier P, Cherrier MV, Martin L, Marchi-Delapierre C, Fontecilla-Camps JC, Ménage S. "Crystallographic snapshots of the reaction of aromatic C-H with O2 catalysed by a protein-bound iron complex." (2010) Nat. Chem. 2, 1069-1076. [UV-vis Absorption/Raman]
  4. Faro AR, Adam V, Carpentier P, Darnault C, Bourgeois D, de Rosny E. "Low-temperature switching by photoinduced protonation in photochromic fluorescent proteins." (2010) Photochem. Photobiol. Sci. 9, 254-262. [UV-vis Absorption/Fluorescence]
  5. Sans JA, Martinez-Criado G, Susini J, Sanz R, Jensen J, Minguez I, Hernandez-Velez M, Labrador A, Carpentier P. "Thermal instability of implanted Mn ions in ZnO." (2010) J. Appl. Phys. 107, 023507. [Raman]

Review or citation

  1. Dierks K, Meyer A, Oberthur D, Rapp G, Einspahr H, Betzel C. "Efficient UV detection of protein crystals enabled by fluorescence excitation at wavelengths longer than 300 nm." (2010) Acta Cryst. F66, 478-484.
  2. Garman EF. "Radiation damage in macromolecular crystallography: what is it and why should we care?" (2010) Acta Cryst. D66, 339-351.
  3. Weik M, Colletier JP. "Temperature-dependent macromolecular X-ray crystallography." (2010) Acta Cryst. D66, 437-446.

2009

Experimental data

  1. Adam V, Carpentier P, Violot S, Lelimousin M, Darnault C, Nienhaus GU, Bourgeois D. "Structural basis of X-ray-induced transient photobleaching in a photoactivatable green fluorescent protein." (2009) J. Am. Chem. Soc. 131, 18063-18065. [(on-line) UV-vis Absorption/Fluorescence/Raman]
  2. Barros T, Royant A, Standfuss J, Dreuw A, Kühlbrandt W. "Crystal structure of plant light-harvesting complex shows the active, energy-transmitting state." (2009) EMBO J. 28, 298-306. [UV-vis Absorption/Fluorescence/TCSPC]
  3. Carpentier P, Violot S, Blanchoin L, Bourgeois D. "Structural basis for the phototoxicity of the fluorescent protein KillerRed." (2009) FEBS Lett. 583, 2839-42. [UV-vis Absorption/Fluorescence]
  4. Durin G, Delaunay A, Darnault C, Heyes DJ, Royant A, Vernède X, Hunter CN, Weik M, Bourgeois D. "Simultaneous measurements of solvent dynamics and functional kinetics in a light-activated enzyme." (2009) Biophys. J. 96, 1902-1910. [UV-vis Absorption/Fluorescence]
  5. Howard-Jones AR, Adam V, Cowley A, Baldwin JE, Bourgeois D. "Cryophotolysis of a caged oxygen compound for use in low temperature biological studies." (2009) Photochem. Photobiol. Sci. 8, 1150-1156. [UV-vis Absorption]
  6. Lafaye C, Iwema T, Carpentier P, Jullian-Binard C, Kroll JS, Collet JF, Serre L. "Biochemical and structural study of the homologues of the thiol-disulfide oxidoreductase DsbA in Neisseria meningitidis." (2009) J. Mol. Biol. 392, 952-966. [Raman]
  7. Lelimousin M, Noirclerc-Savoye M, Lazareno-Saez C, Paetzold B, Le Vot S, Chazal R, Macheboeuf P, Field MJ, Bourgeois D, Royant A. "Intrinsic dynamics in ECFP and Cerulean control fluorescence quantum yield." (2009) Biochemistry 48, 10038-10046. [UV-vis Absorption/Fluorescence/TCSPC]
  8. Macedo S, Pechlaner M, Schmid W, Weik M, Sato K, Dennison C, Djinovic-Carugo K. "Can soaked-in scavengers protect metalloprotein active sites from reduction during data collection?" (2009) J. Synchrotron Rad. 16, 191-204. [on-line UV-vis Absorption]
  9. Mailliet J, Psakis G, Schroeder C, Kaltofen S, Dürrwang U, Hughes J, Essen LO. "Dwelling in the dark: procedures for the crystallography of phytochromes and other photochromic proteins" (2009) Acta Cryst. D65, 1232-1235. [UV-vis Absorption]
  10. McGeehan J, Ravelli RB, Murray JW, Owen RL, Cipriani F, McSweeney S, Weik M, Garman EF. "Colouring cryo-cooled crystals: online microspectrophotometry." (2009) J. Synchrotron Rad. 16, 163-172. [on-line UV-vis Absorption/X-ray-Excited Optical Luminescence (XEOL)]
  11. Moldt J, Pokorny R, Orth C, Linne U, Geisselbrecht Y, Marahiel MA, Essen LO, Batschauer A. "Photoreduction of the folate cofactor in members of the photolyase family." (2009) J. Biol. Chem. 284, 21670-82163. [UV-vis Absorption/Fluorescence]
  12. Pogoryelov D, Yildiz O, Faraldo-Gómez JD, Meier T. "High-resolution structure of the rotor ring of a proton-dependent ATP synthase." (2009) Nat. Struct. Mol. Biol. 16, 1068-1073. [UV-vis Absorption]
  13. Violot S, Carpentier P, Blanchoin L, Bourgeois D. "Reverse pH-dependence of chromophore protonation explains the large stokes shift of the red fluorescent protein mKeima." (2009) J. Am. Chem. Soc. 131, 10356-10357. [UV-vis Absorption/Fluorescence/Raman]

Review or citation

  1. Bourgeois D, Katona G, de Rosny E, Carpentier P. "Raman-assisted x-ray crystallography for the analysis of biomolecules." (2009) Methods Mol. Biol. 544, 253-267.
  2. Bourgeois D, Weik M. "Kinetic protein crystallography: a tool to watch proteins in action." (2009) Crystallography Reviews 15, 87-118.
  3. Garman EF, Nave C. "Radiation damage in protein crystals examined under various conditions by different methods." (2009) J. Synchrotron Rad. 16, 129-132.
  4. Owen RL, Pearson AR, Meents A, Boehler P, Thominet V, Schulze-Briese C. "A new on-axis multimode spectrometer for the macromolecular crystallography beamlines of the Swiss Light Source." (2009) J. Synchrotron Rad. 16, 173-182.
  5. Pearson AR, Owen RL. "Combining X-ray crystallography and single-crystal spectroscopy to probe enzyme mechanisms." (2009) Biochem. Soc. Trans. 37, 378-381.

2008

Experimental data

  1. Adam V, Lelimousin M, Boehme S, Desfonds G, Nienhaus K, Field MJ, Wiedenmann J, McSweeney S, Nienhaus GU, Bourgeois D. "Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations." (2008) Proc. Natl. Acad. Sci. USA 105(47): 18343-18348. [UV-vis Absorption/Fluorescence]
  2. Essen LO, Mailliet J, Hughes J. "The structure of a complete phytochrome sensory module in the Pr ground state." (2008) Proc. Natl. Acad. Sci. USA 105, 14709-14714. [UV-vis Absorption]
  3. Hersleth HP, Hsiao YW, Ryde U, Görbitz CH, Andersson KK. "The crystal structure of peroxymyoglobin generated through cryoradiolytic reduction of myoglobin compound III during data collection." (2008) Biochem. J. 412, 257-264. [UV-vis Absorption]

Review or citation

  1. Coureux PD, Fan ZP, Stojanoff V, Genick UK. "Picometer-scale conformational heterogeneity separates functional from nonfunctional states of a photoreceptor protein." (2008) Structure 16 (6): 863-872
  2. Ellis MJ, Buffey SG, Hough MA, Hasnain SS. "On-line optical and X-ray spectroscopies with crystallography: an integrated approach for determining metalloprotein structures in functionally well defined states." (2008) J. Synchrotron Rad. 15, 433-439.
  3. Hersleth HP, Hsiao YW, Ryde U, Görbitz CH, Andersson KK. "The influence of X-rays on the structural studies of peroxide-derived myoglobin intermediates." (2008) Chem. Biodivers. 5, 2067-2089.
  4. Merlino A, Sica F, Mazzarella L, Zagari A, Vergara A. "Correlation between Raman and X-ray crystallography data of (Pro-Pro-Gly)10." (2008) Biophys. Chem. 137, 24-27.
  5. Strange RW, Feiters MC. "Biological X-ray absorption spectroscopy (BioXAS): a valuable tool for the study of trace elements in the life sciences." (2008) Curr. Opin. Struct. Biol. 18, 609-616.

2007

Experimental data

  1. Carpentier P, Royant A, Ohana J, Bourgeois D. "Advances in spectroscopic methods for biological crystals. Part 2 - Raman spectroscopy." (2007) J. Appl. Crystallogr. 40, 1113-1122. [(on-line) Raman]
  2. Colletier JP, Royant A, Specht A, Sanson B, Nachon F, Masson P, Zaccai G, Sussman JL, Goeldner M, Silman I, Bourgeois D, Weik M. "Use of a 'caged' analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography." (2007) Acta Cryst. D63, 1115-1128. [UV-vis Absorption/on-line actinic]
  3. Katona G, Carpentier P, Nivière V, Amara P, Adam V, Ohana J, Tsanov N, Bourgeois D. "Raman-assisted crystallography reveals End-On peroxide intermediates in a nonheme iron enzyme." (2007) Science 316, 449-453. [Raman]
  4. McGeehan JE, Carpentier P, Royant A, Bourgeois D, Ravelli RBG. "X-ray Radiation-induced damage in DNA monitored by online Raman." (2007) J. Synchrotron Rad. 14, 97-108. [on-line Raman]
  5. Royant A, Carpentier P, Ohana J, McGeehan J, Paetzold B, Noirclerc-Savoye M, Vernède X, Adam V, Bourgeois D. "Advances in spectroscopic methods for biological crystals. Part 1 - Fluorescence lifetime measurements." (2007) J. Appl. Crystallogr. 40, 1105-1112. [Fluorescence/TCSPC]
  6. Schmidt M, Patel A, Zhao Y, Reuter W. "Structural basis for the photochemistry of alpha-phycoerythrocyanin." (2007) Biochemistry 46, 416-423. [UV-vis Absorption]

Review or citation

  1. Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I. "Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography." (2007) J. Synchrotron Rad. 14, 11-23.
  2. Bourgeois D, de Rosny E, Katona G. "La cristallographie cinétique : un outil pour filmer les protéines en action." (2007) Biofutur 280, 48-51.

2006

Experimental data

  1. Marcaida MJ, Schlarb-Ridley BG, Worrall JAR, Wastl J, Evans TJ, Bendall DS, Luisi BF, Howe CJ. "Structure of cytochrome c6A, a novel dithio-cytochrome of Arabidopsis thaliana, and its reactivity with plastocyanin: Implications for function." (2006) J. Mol. Biol. 360, 968-977. [UV-vis Absorption]
  2. Nanao MH, Ravelli RB. "Phasing macromolecular structures with UV-induced structural changes." (2006) Structure 14, 791-800. [on-line actinic]
  3. Sainz G, Jakoncic J, Sieker LC, Stojanoff V, Sanishvili N, Asso M, Bertrand P, Armengaud J, Jouanneau Y. "Structure of a [2Fe-2S] ferredoxin from Rhodobacter capsulatus likely involved in Fe-S cluster biogenesis and conformational changes observed upon reduction." (2006) J. Biol. Inorg. Chem. 11, 235-246. [UV-vis Absorption]
  4. Vernède X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D. "UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops." (2006) Acta Cryst. D62, 253-261. [Fluorescence]
  5. Williams P, Coates L, Mohammed F, Gill R, Erskine P, Bourgeois D, Wood SP, Anthony C, Cooper JB. "The 1.6 A X-ray structure of the unusual c-type cytochrome, cytochrome cL, from the methylotrophic bacterium Methylobacterium extorquens." (2006) J. Mol. Biol. 357, 151-162. [UV-vis Absorption]

Review or citation

  1. Klink BU, Goody RS, Scheidig AJ. "A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction." (2006) Biophys. J. 91, 981-992.

2005

Experimental data

  1. Dubnovitsky AP, Ravelli RBG, Popov AN, Papageorgiou AC. "Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage." (2005) Protein Sci. 14, 1498-1507. [on-line UV-vis Absorption]

Review or citation

  1. Bourgeois D, Royant A. "Advances in kinetic protein crystallography." (2005) Curr. Opin. Struct. Biol. 15, 538-547.
  2. Bourgeois D, Weik M. "New perspectives in kinetic protein crystallography using caged compounds" (2005) in "Dynamic Studies in Biology: Phototriggers, Photoswitches and Caged Biomolecules", edited by M. Goeldner & R. Givens, pp. 410-435. Weinheim: Wiley-VCH.
  3. Johansson T, Oswald C, Pedersen A, Tornroth S, Okvist M, Karlsson BG, Rydstrom J, Krengel U. "X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli." (2005) J. Mol. Biol. 352, 299-312.
  4. Judge RA, Swift K, Gonzalez C. "An ultraviolet fluorescence-based method for identifying and distinguishing protein crystals." (2005) Acta Cryst. D61, 60-66.

2004

Experimental data

  1. Adam V, Royant A, Niviere V, Molina-Heredia FP, Bourgeois D. "Structure of superoxide reductase bound to ferrocyanide and active site expansion upon X-ray-induced photo-reduction." (2004) Structure 12, 1729-1740. [(on-line) UV-vis Absorption]
  2. Dias JM, Alves T, Bonifácio C, Pereira AS, Trincão J, Bourgeois D, Moura I, Romão MJ. "Structural basis for the mechanism of Ca2+ activation of the di-heme cytochrome c peroxidase from Pseudomonas nautica 617." (2004) Structure 12, 961-973. [UV-vis Absorption]
  3. Edman K, Royant A, Larsson G, Jacobson F, Taylor T, van der Spoel D, Landau EM, Pebay-Peyroula E, Neutze R. "Deformation of helix C in the low temperature L-intermediate of bacteriorhodopsin." (2004) J. Biol. Chem. 279, 2147-2158. [UV-vis Absorption]
  4. Jacquamet L, Ohana J, Joly J, Legrand P, Kahn R, Borel F, Pirocchi M, Charrault P, Carpentier P, Ferrer JL. "A new highly integrated sample environment for protein crystallography." (2004) Acta Cryst. D60, 888-894. [Fluorescence]
  5. Kort R, Hellingwerf KJ, Ravelli RBG. "Initial events in the photocycle of photoactive yellow protein." (2004) J. Biol. Chem. 279, 26417-26424. [UV-vis Absorption]
  6. Kort R, Komori H, Adachi S, Miki K, Eker A. "DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction." (2004) Acta Cryst. D60, 1205-1213. [UV-vis Absorption]
  7. Weik M, Vernède X, Royant A, Bourgeois D. "Temperature derivative fluorescence spectroscopy as a tool to study dynamical changes in protein crystals." (2004) Biophys. J. 86, 3176-3185. [Fluorescence]

Review or citation

  1. Neutze R, Huldt G, Hajdu J, van der Spoel D. "Potential impact of an X-ray free electron laser on structural biology." (2004) Rad. Phys. Chem. 71, 905-916.
  2. Pearson AR, Mozzarelli A, Rossi GL. "Microspectrophotometry for structural enzymology." (2004) Curr. Opin. Struct. Biol. 14, 656-662.
  3. Powe AM, Fletcher KA, St Luce NN, Lowry M, Neal S, McCarroll ME, Oldham PB, McGown LB, Warner IM. "Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry." (2004) Anal. Chem. 76, 4614-4634.

2003

Experimental data

  1. Aragão D, Macedo S, Mitchell EP, Romão CV, Liu MY, Frazão C, Saraiva LM, Xavier AV, LeGall J, van Dongen WMAM, Hagen WR, Teixeira M, Carrondo MA, Lindley P. "Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation." (2003) J. Biol. Inorg. Chem. 8, 540-548. [UV-vis Absorption]
  2. Fioravanti E, Haouz A, Ursby T, Munier-Lehmann H, Delarue M, Bourgeois D. "Mycobacterium tuberculosis thymidylate kinase: Structural studies of intermediates along the reaction pathway." (2003) J. Mol. Biol. 327,1077-1092. [Fluorescence]
  3. Kort R, Ravelli RBG, Schotte F, Bourgeois D, Crielaard W, Hellingwerf K J, Wulff M. "Characterization of photocycle intermediates in crystalline photoactive yellow protein." (2003) Photochem. Photobiol. 78, 131-137. [UV-vis Absorption]

2002

Experimental data

  1. Bourgeois D, Vernède X, Adam V, Fioravanti E, Ursby T. "A microspectrophotometer for UV-visible absorption and fluorescence studies of protein crystals." (2002) J. Appl. Crystallogr. 35, 319-326. [UV-vis Absorption/Fluorescence]
  2. Murray J, Garman E. "Investigation of possible free-radical scavengers and metrics for radiation damage in protein cryocrystallography." (2002) J. Synchrotron Rad. 9, 347-354. [UV-vis Absorption]
  3. Okada T, Fujiyoshi Y, Silow M, Navarro J, Landau EM, Shichida Y. "Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography." (2002) Proc. Natl. Acad. Sci. USA 99, 5982-5987. [UV-vis Absorption]
  4. Ursby T, Weik M, Fioravanti E, Delarue M, Goeldner M, Bourgeois D. "Cryophotolysis of caged compounds: a technique for trapping intermediate states in protein crystals." (2002) Acta Cryst. D58, 607-614. [UV-vis Absorption]
  5. Weik M, Bergès J, Raves ML, Gros P, McSweeney S, Silman I, Sussman JL, Houée-Levin C, Ravelli RBG. "Evidence for the formation of disulfide radicals in protein crystals upon X-ray irradiation." (2002) J. Synchrotron Rad. 9, 342-346. [UV-vis Absorption]

Review or citation

  1. Prasad GS, Wahlberg M, Sridhar V, Sundaresan V, Yamaguchi M, Hatefi Y, Stout CD. "Crystal structures of transhydrogenase domain I with and without bound NADH." (2002) Biochemistry 41, 12745-12754.

2001

Experimental data

  1. Nicolet Y, de Lacey AL, Vernède X, Fernandez VM, Hatchikian EC, Fontecilla-Camps JC. "Crystallographic and FTIR spectroscopic evidence of changes in Fe coordination upon reduction of the active site of the Fe-only hydrogenase from Desulfovibrio desulfuricans." (2001) J. Am. Chem. Soc. 123, 1596-1601. [UV-vis Absorption]
  2. Specht A, Ursby T, Weik M, Peng L, Kroon J, Bourgeois D, Goeldner M. "Cryophotolysis of ortho-nitrobenzyl derivatives of enzyme ligands for the potential kinetic crystallography of macromolecules." (2001) Chembiochem 2, 845-848. [UV-vis Absorption]

2000

Experimental data

  1. Royant A, Edman K, Ursby T, Pebay-Peyroula E, Landau EM, Neutze R. "Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin." (2000) Nature 406, 645-648. [UV-vis Absorption]

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