Structural studies using online spectrophotometry (in User Guide)



  • Structure of Superoxide Reductase Bound to Ferrocyanide and Active Site Expansion upon X-Ray-Induced Photo-Reduction.
    Adam, V., Royant, A., Niviere, V., Molina-Heredia, F.P. & Bourgeois, D.
    Structure, Vol 12,1729-1740, 2004


  • Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage.
    Dubnovitsky, A.P., Ravelli, R.B.G., Popov, A.N. & Papageorgiou, A.C.
    Protein Science, Vol 14, 1-10, 2005


  • High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle.
    Edman, K., Nollert, P., Royant, A., Belrhali, H., Pebay-Peyroula, E., Hajdu, J., Neutze, R. & Landau, E.M.
    Nature, Vol 401, 748, 1999


  • The catalytic pathway of horseradish peroxidase at high resolution.
    Berglund, G.I., Carlsson, G.H., Smith, A.T., Szoke, H., Henriksen, A. & Hajdu, J.
    Nature, Vol 417, 463-468, 2002


  • Evidence for the formation of disulfide radicals in protein crystals upon X-ray irradiation.
    Weik, M., Bergès, J., Raves, M.L., Gros, P., McSweeney, S., Silman, I., Sussman, J.L., Houée-Levin, C. & Ravelli, R.B.G.
    Journal of Synchrotron Radiation, Vol 9, 342-346, 2002


    Technical and method development


  • A microspectrophotometer for UV-visible absorption and fluorescence studies of protein crystals.
    Bourgeois, D., X. Vernede, V. Adam, E. Fioravanti, and T. Ursby.
    J. Appl. Crystallogr, Vol 35, 319–326, 2002



  • Temperature derivative fluorescence spectroscopy as a tool to study dynamical changes in protein crystals.
    Weik M, Vernede X, Royant A, Bourgeois D.
    Biophys J., Vol 86, 3176-85, 2004



  • Advances in kinetic protein crystallography.
    Bourgeois D, Royant A.
    Curr Opin Struct Biol., Vol 15, 538-47, 2005



  • UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops.
    Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D.
    Acta Cryst.D, Vol 62, 253-61, 2006



  • Microspectrophotometry for structural enzymology
    Pearson, A.R., Mozzarelli, A. and Rossi, G.L.
    Curr Opin Struct Biol., Vol 14, 656-62, 2004


    Selected papers



  • Investigation of possible free-radical scavengers and metrics for radiation damage in protein cryocrystallography
    J. Murray and E. Garman
    J. Synchrotron Rad., Vol 9, 347-354, 2002



  • X-ray induced reduction of the crystal of high-molecular-weight cytochrome c revealed by microspectrophotometry
    M. Sato, N. Shibata, Y. Morimoto, Y. Takayama, K. Ozawa, H. Akutsu, Y. Higuchi and N. Yasuoka
    J. Synchrotron Rad., Vol 11, 113-116, 2004



  • Characterization of photocycle intermediates in crystalline photoactive yellow protein.
    Kort R, Ravelli RB, Schotte F, Bourgeois D, Crielaard W, Hellingwerf KJ, Wulff M.
    Photochem Photobiol., Vol 78, 131-137, 2003



  • Absorption spectroscopy of three-dimensional bacteriorhodopsin crystals at cryogenic temperatures: effects of altered hydration
    E. Portuondo-Campa, S. Schenkl, M. Dolder, M. Chergui, E. M. Landau and S. Haacke
    Acta Cryst.D, Vol D62, 368-374, 2006



  • Phasing macromolecular structures with UV-induced structural changes.
    Nanao MH, Ravelli RB.
    Structure, Vol 14, 791-800, 2006