EMBO Practical Course Strategies in Macromolecular Structure Determination at 3rd Generation Synchrotron Sources

17th - 24 th June 2003, ESRF & EMBL Outstation, Grenoble, France

Introduction

Synchrotron radiation has become a important tool in the determination of the 3-dimensional structure of biological macromolecules. The objectives of this practical course are to give scientists a sturdy foundation in the different methods in macromolecular crystallography specifically employed at synchrotron radiation facilities - especially data collection techniques and phasing methods with anomalous scattering (MAD & SAD methods), atomic resolution studies and automation.

The practical course will consist of:

• Lectures of the theoretical principles and their applications,
• Hands-on tutorials using programs for data integration, substructure determination and phase calculations,
• Practicals on MAD & fixed wavelength beamlines at the ESRF.

The lecture topics will cover:

• General theory and application of anomalous scattering to macromolecular crystallography
• Data collection, integration & processing
• Substructure determination via Patterson and direct methods
• Calculation of experimental phases
• Model building, High resolution studies & Automation
• Novel Methods

The deadline for applications is now closed.

Please note: We are no longer permitting candidates to bring their own samples for data colletion. Our sincere apologies for any inconvenience.