Overview
BM29 is the dedicated BioSAXS beamline at the ESRF, primarily used for the characterisation of biological macromolecules in solution. Following the ESRF-EBS upgrade project, BM29 has been significantly upgraded and refurbished.
Beamline specifications
| Energy | Standard 12.5 keV (7-15 keV available) |
| q-range | 0.025 - 6 nm-1 |
| Beam size at sample | ~ 200 um x 100 um |
| Flux at sample | 1.4 x 1013 (12.5 keV, 200 mA) |
| Sample environments | Quartz glass capillary, microfludic chips |
| Sample loading | Automated sample changer, on-line SEC, microfluidic setup, manual load |
| Detector | Pilatus3 2M in vacuum |
| Monochromator | Double multilayer monochromator (energy band pass ~ 10-2) |
| Mirror | 4 mrad torodial mirror, 1.1 m long |
Sample exposure units and sample loading
SEU2A - standard exposure unit
- Typical sample environment is a 1 mm quartz capillary.
- Sample exposure temperature from 4°C to 50°C.
- Compatible with automated sample changer robot and HPLC set up.
- Sample changer
- Minimum/maximum sample volume - 10 - 100 ul
- Sample storage temperature range - 5 - 50 ºC
- PCR strips and 96 well plates available to be used in sample changer.
- HPLC
- Minimum/maximum sample volume - 5 - 100 ul
- Sample storage temperature range - 5 - 25 ºC
- Manual injection port also available - 5 ml maximum.
- Sample changer
- Fully integrated with BSXCuBE3.
SEU-2B - Microfluidic compatible sample environments
- Microfludic sample environment.
- Mixing experiments
- Gel chips
- In-vacuum x/y/z piezo stages.
partners
European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.