Cryostream
All MX group beamlines are equipped with Oxford Cryosystems 700 series cryostreams. They are mounted on a pneumatic slider for in/out positioning of the cryostream (controlled via the exes interface) to ease manual mounting of samples and used automatically by the sample changer when in use.
The cryostream should already be running at 100K when you arrive. If you wish to change the temperature during your experiment please feel free to do so but please reset it to 100K before you leave.
The dewar for the cryostream should be automatically refilled. You can monitor the status of the cryostream and it's dewar using the cryospy software on each beamline control PC. If necessary the LN2 dewar can be refilled manually by selecting "Manual" on the timer box. Always put the switch back to automatic when you have finished!!
The cryostream is easily aligned using the varibeam support and alignment tool attached to the support stand. If you wish to align the cryostream please ensure that you check the translation of the cryostream so that when the software drives the cryostream in, it doesn't hit the backstop. To do this, align the cryostream with the alignment tool to a centred pin with the backstop at the out position. Remove the alignment tool and ensure that the cryostream is in the IN position. Bring the backstop in and make sure that it doesn't collide with the cryostream (use the translation on the varibeam support to set this correctly). Ensure the cryostream is as close as you can get to the sample without hitting the collimator/backstop.
The importance of cryostream distance from sample
Test data were collected from a single trypsin crystal supported in a nylon loop with the cryostream aligned on the sample but the cryostream at 3 different distances from the sample. Four 180o (multiplicity ~7) data sets were collected with 1o oscillations, 3 passes, 1 second exposures with cryostream to sample distances of 5mm, 10mm, 15mm and 5mm again (to account for radiation damage, etc).
We do not observe such marked effects when using litholoops.
Cryostream positions:
2 data sets were also collected at 5mm and 15mm cryostream to sample distances with 10s exposures with 10 passes with the beam attenuated to approximately a tenth of the prior data sets.
A summary of the statistics for these data sets are shown below.
Conclusions we can draw from this are that it is preferable to place the cryostream to within 5mm of the sample while collecting data (and certainly closer than 10mm), and this is especially important when collecting data with short exposure times.
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-mattset 1; 1o 1sec 3 passes; 180o-
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# Cryostream 5mm from xtal #
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N 1/d^2 Dmin(A) Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
1 0.0346 5.38 0.027 0.024 0.027 0.013 562 6758. 312.7 21.6 348. 47.3 4974 804 242 -0.028 1140
2 0.0692 3.80 0.026 0.022 0.026 0.013 1153 13651. 637.3 21.4 692. 50.6 9606 1432 279 -0.032 2326
3 0.1038 3.10 0.027 0.023 0.027 0.011 1536 11393. 540.8 21.1 573. 51.8 12732 1823 287 -0.030 3058
4 0.1384 2.69 0.029 0.025 0.027 0.012 1834 5987. 299.5 20.0 304. 50.5 15100 2119 285 -0.030 3585
5 0.1730 2.40 0.030 0.026 0.028 0.014 2103 4312. 216.2 19.9 221. 49.1 17207 2395 292 -0.027 4157
6 0.2076 2.19 0.031 0.027 0.028 0.013 2353 3745. 187.4 20.0 196. 47.9 19106 2642 289 -0.028 4484
7 0.2422 2.03 0.034 0.031 0.029 0.015 2556 3256. 179.3 18.2 183. 44.2 20589 2837 281 -0.031 4837
8 0.2768 1.90 0.038 0.034 0.030 0.016 2777 2438. 143.0 17.1 145. 41.1 22325 3064 284 -0.029 5313
9 0.3114 1.79 0.045 0.040 0.030 0.018 2934 1605. 109.1 14.7 103. 36.5 23452 3221 282 -0.027 5589
10 0.3460 1.70 0.053 0.048 0.031 0.023 3112 1213. 95.6 12.7 83. 33.6 24514 3388 271 -0.028 5748
Overall: 0.031 0.027 0.031 0.014 20920 4332. 272.2 15.9 232. 43.8 169605 23725 2792 -0.030 40237
Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
#############################
# Cryostream 10mm from xtal #
#############################
N 1/d^2 Dmin(A) Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
1 0.0346 5.38 0.037 0.033 0.037 0.018 563 6509. 456.2 14.3 410. 37.2 5037 807 245 -0.035 1117
2 0.0692 3.80 0.038 0.034 0.038 0.016 1156 13176. 937.6 14.1 812. 40.2 9769 1438 284 -0.036 2333
3 0.1038 3.10 0.037 0.034 0.037 0.017 1535 10786. 750.8 14.4 659. 41.3 12814 1822 287 -0.032 3097
4 0.1384 2.69 0.038 0.035 0.038 0.016 1836 5617. 395.7 14.2 346. 40.3 15182 2123 287 -0.030 3558
5 0.1730 2.40 0.040 0.036 0.038 0.017 2103 4030. 291.5 13.8 251. 39.3 17250 2394 291 -0.034 4149
6 0.2076 2.19 0.040 0.036 0.038 0.017 2353 3494. 232.8 15.0 221. 38.4 19165 2643 290 -0.029 4500
7 0.2422 2.03 0.044 0.040 0.039 0.019 2561 3019. 228.4 13.2 205. 35.7 20655 2842 281 -0.030 4857
8 0.2768 1.90 0.048 0.044 0.040 0.020 2776 2261. 178.0 12.7 162. 33.3 22315 3063 284 -0.025 5285
9 0.3114 1.79 0.056 0.050 0.040 0.023 2935 1482. 131.4 11.3 115. 29.5 23456 3221 281 -0.020 5531
10 0.3460 1.70 0.064 0.058 0.041 0.027 3112 1116. 109.6 10.2 94. 27.1 24521 3389 272 -0.022 5811
Overall: 0.041 0.037 0.041 0.018 20930 4093. 377.3 10.8 265. 35.1 170164 23742 2802 -0.031 40238
Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
#############################
# Cryostream 15mm from xtal #
#############################
N 1/d^2 Dmin(A) Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
1 0.0346 5.38 0.100 0.097 0.100 0.045 565 7744. 1486.5 5.2 916. 19.6 5057 811 246 -0.038 1228
2 0.0692 3.80 0.099 0.094 0.099 0.046 1159 15371. 2952.5 5.2 1805. 21.0 9797 1445 286 -0.028 2481
3 0.1038 3.10 0.099 0.093 0.099 0.043 1538 12552. 2317.6 5.4 1474. 21.3 12825 1825 287 -0.034 3292
4 0.1384 2.69 0.097 0.093 0.098 0.044 1836 6465. 1166.8 5.5 768. 20.7 15166 2123 287 -0.041 3850
5 0.1730 2.40 0.097 0.093 0.098 0.042 2111 4604. 824.1 5.6 553. 20.1 17313 2404 293 -0.026 4550
6 0.2076 2.19 0.098 0.093 0.098 0.042 2353 3956. 713.4 5.5 485. 19.6 19141 2644 291 -0.028 4816
7 0.2422 2.03 0.100 0.095 0.098 0.043 2562 3441. 629.0 5.5 458. 17.9 20666 2844 282 -0.039 5169
8 0.2768 1.90 0.101 0.095 0.099 0.043 2783 2560. 469.0 5.5 362. 16.6 22361 3066 280 -0.040 5663
9 0.3114 1.79 0.107 0.102 0.099 0.046 2941 1654. 322.5 5.1 255. 14.6 23505 3228 282 -0.021 6032
10 0.3460 1.70 0.111 0.103 0.099 0.050 3117 1244. 243.2 5.1 209. 13.3 24573 3393 270 -0.026 6261
Overall: 0.099 0.094 0.099 0.044 20965 4706. 1148.1 4.1 589. 17.8 170404 23783 2804 -0.032 43342
Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
##################################
# Cryostream 5mm from xtal again #
##################################
N 1/d^2 Dmin(A) Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
1 0.0346 5.38 0.025 0.022 0.025 0.013 565 6164. 274.4 22.5 307. 47.0 5071 811 246 -0.028 1153
2 0.0692 3.80 0.025 0.021 0.025 0.011 1158 12361. 565.5 21.9 608. 50.5 9798 1442 287 -0.030 2347
3 0.1038 3.10 0.025 0.021 0.025 0.010 1536 9951. 431.2 23.1 487. 51.4 12822 1823 287 -0.029 3047
4 0.1384 2.69 0.027 0.023 0.025 0.011 1838 5094. 241.9 21.1 252. 50.0 15193 2125 287 -0.030 3554
5 0.1730 2.40 0.029 0.024 0.026 0.012 2105 3635. 175.3 20.7 182. 48.6 17268 2398 293 -0.029 4125
6 0.2076 2.19 0.030 0.026 0.026 0.013 2356 3119. 149.5 20.9 159. 47.3 19180 2647 291 -0.027 4433
7 0.2422 2.03 0.034 0.030 0.027 0.015 2563 2698. 144.8 18.6 148. 43.7 20654 2843 280 -0.029 4740
8 0.2768 1.90 0.040 0.035 0.028 0.016 2776 2000. 122.8 16.3 117. 40.4 22292 3061 282 -0.025 5248
9 0.3114 1.79 0.049 0.043 0.029 0.020 2939 1304. 94.4 13.8 84. 35.5 23483 3226 282 -0.022 5463
10 0.3460 1.70 0.059 0.053 0.030 0.025 3114 972. 84.1 11.6 68. 32.2 24504 3392 273 -0.022 5720
Overall: 0.030 0.026 0.030 0.013 20950 3728. 228.6 16.3 194. 43.1 170265 23768 2808 -0.028 39830
Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
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-mattset 3; 1o 10sec 10 passes; 180o-
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############################
# Cryostream 5mm from xtal #
############################
N 1/d^2 Dmin(A) Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
1 0.0346 5.38 0.026 0.024 0.026 0.013 570 3520. 173.1 20.3 182. 45.0 5190 823 253 -0.017 1078
2 0.0692 3.80 0.025 0.021 0.025 0.010 1162 6832. 325.5 21.0 351. 48.2 9988 1454 292 -0.024 2269
3 0.1038 3.10 0.024 0.020 0.024 0.010 1534 5344. 223.4 23.9 276. 48.1 12880 1822 288 -0.020 2837
4 0.1384 2.69 0.026 0.024 0.025 0.011 1838 2687. 118.4 22.7 142. 45.9 15215 2125 287 -0.023 3277
5 0.1730 2.40 0.030 0.026 0.025 0.012 2106 1902. 88.2 21.6 103. 43.9 17310 2399 293 -0.020 3807
6 0.2076 2.19 0.032 0.029 0.026 0.013 2358 1623. 79.1 20.5 90. 42.2 19214 2649 291 -0.017 4082
7 0.2422 2.03 0.038 0.035 0.027 0.016 2559 1397. 80.4 17.4 85. 38.5 20660 2841 282 -0.018 4406
8 0.2768 1.90 0.048 0.043 0.029 0.019 2783 1030. 71.6 14.4 69. 34.7 22369 3066 280 -0.018 4829
9 0.3114 1.79 0.064 0.058 0.031 0.026 2937 663. 61.2 10.8 52. 28.9 23490 3225 283 -0.011 5031
10 0.3460 1.70 0.082 0.074 0.032 0.033 3116 492. 56.3 8.7 44. 25.2 24583 3396 272 -0.012 5310
Overall: 0.032 0.029 0.032 0.014 20963 1989. 126.6 15.7 113. 38.0 170899 23800 2821 -0.020 36926
Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
#############################
# Cryostream 15mm from xtal #
#############################
N 1/d^2 Dmin(A) Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
1 0.0346 5.38 0.056 0.052 0.056 0.024 571 3762. 392.5 9.6 288. 30.2 5198 825 254 -0.020 1306
2 0.0692 3.80 0.053 0.050 0.054 0.022 1162 7280. 740.7 9.8 556. 32.4 9982 1453 291 -0.018 2660
3 0.1038 3.10 0.052 0.048 0.053 0.023 1541 5660. 542.9 10.4 434. 32.4 12924 1830 289 -0.016 3378
4 0.1384 2.69 0.055 0.051 0.054 0.024 1838 2834. 284.1 10.0 222. 30.8 15210 2125 287 -0.016 3917
5 0.1730 2.40 0.054 0.051 0.054 0.023 2114 2009. 188.9 10.6 161. 29.4 17352 2406 292 -0.015 4649
6 0.2076 2.19 0.058 0.054 0.054 0.025 2356 1715. 172.6 9.9 142. 28.2 19191 2648 292 -0.016 4903
7 0.2422 2.03 0.062 0.058 0.055 0.026 2566 1483. 154.3 9.6 135. 25.6 20721 2848 282 -0.012 5290
8 0.2768 1.90 0.070 0.064 0.056 0.029 2783 1091. 125.9 8.7 109. 23.0 22381 3068 282 -0.014 5735
9 0.3114 1.79 0.082 0.074 0.057 0.034 2946 701. 87.4 8.0 82. 19.1 23545 3233 282 -0.009 6232
10 0.3460 1.70 0.099 0.088 0.059 0.041 3113 521. 76.1 6.8 72. 16.5 24571 3391 271 -0.012 6362
Overall: 0.059 0.054 0.059 0.025 20990 2109. 282.7 7.5 178. 25.3 171075 23827 2822 -0.016 44432
Rmrg Rfull Rcum Ranom Nanom Av_I SIGMA I/sigma sd Mn(I)/sd Nmeas Nref Ncent FRCBIAS Nbias
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