BM29

Synopsis

The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.

Disciplines

• Life Sciences
• Chemistry
• Medicine

Applications

• Structural biology
• Pharmaceuticals

Techniques

• BioSAXS - small-angle X-ray scattering (proteins/DNA)
• SAXS - small-angle X-ray scattering

Sample environments

• Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
• HPLC (high performance liquid chromatography) system can be used in parallel with sample changer

Detectors

• Pilatus3 2M in-vac

Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.